Article

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell lines

2024-08-06

Abstract excerpt

Hypoxia is common in breast tumours and is linked to therapy resistance and advanced disease. To understand hypoxia-driven breast cancer progression, RT-qPCR quantifies transcriptional changes important for malignant development. Reference genes (RGs) are endogenous RT-qPCR controls used to normalise mRNA levels, allowing accurate assessment of transcriptional changes. However, hypoxia reprograms transcription and...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 25a9aeb8-b288-5724-8010-c91c034ea9c8
DOI: 10.1101/2024.08.02.606329

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Identification of robust RT-qPCR reference genes for studying changes in gene expression in response to hypoxia in breast cancer cell linesDOI 10.1101/2024.08.02.606329Read

Selecting Suitable Reference Genes for qPCR Normalization: A Comprehensive Analysis in MCF-7 Breast Cancer Cell LineSemantic proximity Read

Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell lineSemantic proximity Read

Genome Based Search for Reference Genes for Gene Expression Analysis in Oral Cancer: a Data Science Driven ApproachSemantic proximity Read

Normalization Strategy for Selection of Reference Genes for RT-qPCR Analysis in Left Ventricles of Failing Human HeartsSemantic proximity Read

Identification of valid reference genes for circadian gene-expression studies in human mammary epithelial cellsSemantic proximity Read

RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancersSemantic proximity Read

Genes commonly upregulated by hypoxia in human breast cancer cells MCF-7 and MDA-MB-231Semantic proximity Read

Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancerSemantic proximity Read

Hypoxia Classifier for Transcriptome DatasetsSemantic proximity Read

Hypoxia classifier for transcriptome datasetsSemantic proximity Read

Hypoxanthine Phosphoribosyl Transferase 1 Is Upregulated, Predicts Clinical Outcome and Controls Gene Expression in Breast CancerSemantic proximity Read

Validating Reference Gene Expression Stability in Human Ovarian Follicles, Oocytes, Cumulus Cells, Ovarian Medulla, and Ovarian Cortex TissueSemantic proximity Read