Article

An efficient method for immortalization of amniocytes with chromosome abnormalities for renewable application of rare disease sources

2019-11-25

Abstract excerpt

<h4>Objective: </h4> We describe a new method of immortalizing amniocytes with chromosome abnormalities to generate renewable resources for studying rare diseases. <h4>Methods:</h4> Three methods were investigated by randomly dividing 180 cases of adherent amniocytes into groups A, B, and C. Group A cells were digested with 0.25% trypsin for 10, 20, 30, 60, 90, and 120 mins. Group B and C cells were digested with...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 301258ce-c8c1-5214-985b-48fd80b25d47
DOI: 10.21203/rs.2.17757/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Click a neighbor to travelStep 1 · 12 closest

An efficient method for immortalization of amniocytes with chromosome abnormalities for renewable application of rare disease sourcesDOI 10.21203/rs.2.17757/v1Read

Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and ChallengesSemantic proximity Read

Establishment and Characterization of Immortalized Human Amniotic Epithelial CellsSemantic proximity Read

Immortality of cell lines: challenges and advantages of establishmentSemantic proximity Read

Stem Cells in Amniotic Fluid as New Tools to Study Human Genetic DiseasesSemantic proximity Read

Practical Use of Immortalized Cells in Medicine: Current Advances and Future Perspectives.Semantic proximity Read

RPE Differentiation is a Selective Barrier Against Aneuploid hESCSemantic proximity Read

Review of hTERT-Immortalized Cells: How to Assess Immortality and Confirm IdentitySemantic proximity Read

Cultured human amniocytes express hTERT, which is distributed between nucleus and cytoplasm and is secreted in extracellular vesiclesSemantic proximity Read

An efficient method for immortalizing mouse embryonic fibroblastsSemantic proximity Read

Use of Human Amnion Epithelial Cells as a Feeder Layer to Support Undifferentiated Growth of Mouse Embryonic Stem CellsSemantic proximity Read

An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cellsSemantic proximity Read

Immortalized erythroid cells as a novel frontier for in vitro blood production: current approaches and potential clinical applicationSemantic proximity Read