Article

Absolute quantification of rare gene targets in limited samples using crude lysate and ddPCR

2024-03-28

Abstract excerpt

<h4>Background</h4> Accurate quantification of rare genes from limited clinical samples is crucial for research purposes but is technically challenging. In particular, nucleic acid extraction for quantification of gene targets may lead to target loss. Here, we report the development and validation of a novel crude lysate ddPCR assay for the absolute quantification of rare genes, TRECs in our case, from infrequent...

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Literature Corpus work: 28ec3f7a-b570-5b53-b140-ff6abe5bc797
DOI: 10.1101/2024.03.27.586936

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