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Article

Direct Quantification of Cell-Free, Circulating DNA from Unpurified Plasma

3 Mar 2014

Abstract excerpt

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 and 222 bp...

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PMID: 24595313
DOI: 10.1371/journal.pone.0087838

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