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Hydroponic Cultivation of Nicotiana benthamiana: A Stepwise Protocol for Biomass Production in Molecular Farming

2025-09-06

Abstract excerpt

This protocol outlines an efficient hydroponic system for cultivating Nicotiana benthamiana plants as a platform for recombinant protein production. Two hydroponic systems, Deep Water Culture (DWC) and Ebb and Flow (E & F), were compared with conventional tissue culture methods, focusing on plant growth and leaf biomass accumulation. The finding show that hydroponic cultivation yields a ~20-fold increase in leaf b...

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Literature Corpus work: 272dcc9e-016a-5176-8d88-f7b0b1ea6220
DOI: 10.14293/pr2199.001959.v1

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