Article

A Simple Inexpensive Alkaline Method for DNA Extraction from Environmental Samples for PCR Surveillance

2023-11-30

Abstract excerpt

DNA extraction for downstream molecular diagnostic applications can be an expensive, time-consuming process. We devised a method to quickly extract genomic DNA from environmental samples based on sodium hydroxide lysis of cells with or without capture by magnetic beads, for subsequent PCR or quantitative PCR. The final DNA extraction method using NaOH is extremely low-cost and can be completed in 10 minutes at roo...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1d6a47f7-e7bc-5adb-8570-8f76aff03dfd
DOI: 10.20944/preprints202311.2000.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A Simple Inexpensive Alkaline Method for DNA Extraction from Environmental Samples for PCR SurveillanceDOI 10.20944/preprints202311.2000.v1Read

Portable In-Field DNA Sequencing for Rapid Detection of Pathogens and Antimicrobial Resistance: A Proof-of-Concept StudySemantic proximity Read

Impact of Sample Type and DNA Isolation Procedure on Genomic Inference of Microbiome CompositionSemantic proximity Read

A simple, cost-effective and automation-friendly direct PCR approach for bacterial community analysisSemantic proximity Read

An optimized DNA extraction and purification method from dairy manure compost for genetic diversity analysis.Semantic proximity Read

Improved DNA Extraction and Amplification Strategy for 16S rRNA Gene Amplicon-Based Microbiome StudiesSemantic proximity Read

Sensitive and rapid detection of pathogenic bacteria from urine samples using multiplex recombinase polymerase amplificationSemantic proximity Read

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal VirusesSemantic proximity Read

A rapid ionic liquid-based DNA extraction method for molecular diagnostics of urinary tract infectionsSemantic proximity Read

Direct PCR offers a fast and reliable alternative to conventional DNA isolation methods for animal gut microbiomesSemantic proximity Read

Method Optimization for Fecal Sample Collection and Fecal DNA ExtractionSemantic proximity Read

Redistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beadsSemantic proximity Read

Viability Differentiation Improves the Diagnostic Potential of 16S rRNA Gene Sequencing in Ready-to-Eat Meat ManufacturingSemantic proximity Read