Article

Tuning Cell-free Composition Controls the Time-delay, Dynamics, and Productivity of TX-TL Expression

2021-04-02

Abstract excerpt

The composition of TX-TL cell-free expression systems are adjusted by adding macromolecular crowding agents and salts. However, the effects of these cosolutes on the dynamics of individual gene expression processes have not been systematically quantified. Here, we carry out kinetic mRNA and protein level measurements on libraries of genetic constructs using the common cosolutes PEG-8000, Ficoll-400, and magnesium...

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Literature Corpus work: 1b72cae0-050e-519d-a3e7-6bcb5acad03b
DOI: 10.1101/2021.04.02.438196

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Tuning Cell-free Composition Controls the Time-delay, Dynamics, and Productivity of TX-TL ExpressionDOI 10.1101/2021.04.02.438196Read

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