Article

Combinatorial perturbation sequencing on single cells using microwell-based droplet random pairing

2022-08-05

Abstract excerpt

Combinatorial drug therapy reduces drug resistance and disease relapse, but informed drug combinations are lacking due to the high scale of possible combinations and the relatively simple phenotyping strategies. Here we report combinatorial perturbation sequencing (CP-seq) on single cells using microwell-base droplet random pairing. CP-seq uses oligonucleotides to barcode drugs, encapsulates drugs and cells in sep...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 16378681-9ea4-5a60-9476-c3c9e7a5472a
DOI: 10.1101/2022.08.03.502265

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Combinatorial perturbation sequencing on single cells using microwell-based droplet random pairingDOI 10.1101/2022.08.03.502265Read

Combi-Seq: Multiplexed transcriptome-based profiling of drug combinations using deterministic barcoding in single-cell dropletsSemantic proximity Read

Ultra-high throughput single-cell RNA sequencing by combinatorial fluidic indexingSemantic proximity Read

Functional drug screening of tumor organoids on an Active-Matrix Digital Microfluidic Chip for cancer precision medicineSemantic proximity Read

Droplet microfluidics based combinatorial indexing for massive-scale 5′-end single-cell RNA sequencingSemantic proximity Read

Phenotypic drug profiling in droplet microfluidics for better targeting of drug-resistant tumors.Semantic proximity Read

Microfluidics-free single-cell genomics with templated emulsificationSemantic proximity Read

Simple droplet microfluidics platform for drug screening on cancer spheroidsSemantic proximity Read

A patient-specific lung cancer assembloid model with heterogeneous tumor microenvironmentsSemantic proximity Read

Droplet microfluidics forward for tracing target cells at single-cell transcriptome resolutionSemantic proximity Read

Accurate estimation of molecular counts in droplet-based single-cell RNA-seq experimentsSemantic proximity Read

Quantitative and multiplexed chemical-genetic phenotyping in mammalian cells with QMAP-Seq.Semantic proximity Read

Droplet barcoding for massively parallel single-molecule deep sequencing.Semantic proximity Read