Isolation of protein N-terminal peptides by charge-mediated position-selective enrichment using strong cation exchange chromatography

Abstract excerpt

<h4>ABSTRACT</h4> We developed a simple and rapid method to enrich protein N-terminal peptides, in which the protease TrypN is first employed to generate protein N-terminal peptides without Lys or Arg and internal peptides with two positive charges at their N-termini, and then the N-terminal peptides are separated from the internal peptides by means of CHArge-Mediated Position-selective (CHAMP) enrichment using s...

Topics

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• Advanced Proteomics Techniques and Applications
• Cell Membrane
• HEK293 Cells
• Machine Learning in Bioinformatics
• Mutation
• Peptidase Inhibition and Analysis
• RNA and protein synthesis mechanisms
• Ubiquitin and proteasome pathways

Identifiers and source

Literature Corpus work

15bd07f1-c75c-531c-916c-80b7b085df56

DOI

10.1101/2020.06.15.153684