Article

An isothermal calorimetry assay for determining steady state kinetic and enzyme inhibition parameters for SARS-CoV-2 3CL-protease

2024-01-31

Abstract excerpt

<h4>ABSTRACT</h4> This manuscript describes the application of Isothermal Titration Calorimetry (ITC) to characterize the kinetics of 3CL pro from the Severe Acute Respiratory Syndrome CoronaVirus-2 (SARS-CoV-2) and its inhibition by Ensitrelvir, a known non-covalent inhibitor. 3CL pro is the main protease that plays a crucial role of producing the whole array of proteins necessary for the viral infection that...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1124b9a1-fb19-5e21-8370-0b4c7b829b39
DOI: 10.1101/2024.01.31.578159

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An isothermal calorimetry assay for determining steady state kinetic and enzyme inhibition parameters for SARS-CoV-2 3CL-proteaseDOI 10.1101/2024.01.31.578159Read

Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.Semantic proximity Read

Orthogonal dual reporter-based gain-of-signal assay for probing SARS-CoV-2 3CL protease activity in living cells: inhibitor identification and mutation investigation.Semantic proximity Read

The in-vitro effect of famotidine on SARS-CoV-2 proteases and virus replicationSemantic proximity Read

Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays.Semantic proximity Read

Identification of novel proteolytically inactive mutations in coronavirus 3C-like protease using a combined approach.Semantic proximity Read

Enhanced Expression and Solubility of Main Protease (Mpro) of SARS-CoV-2 from E. ColiSemantic proximity Read

Discovery of Potent Triple Inhibitors of Both SARS-CoV-2 Proteases and Human Cathepsin LSemantic proximity Read

Potency Prediction of Covalent Inhibitors against SARS-CoV-2 3CL-like Protease and Multiple Mutants by Multiscale Simulations.Semantic proximity Read

Allosteric Regulation of SARS-CoV-2 Protease: Towards Informed Structure-Based Drug DiscoverySemantic proximity Read

Pro108Ser mutation of SARS-CoV-2 3CLpro reduces the enzyme activity and ameliorates the clinical severity of COVID-19Semantic proximity Read

BRET-based self-cleaving biosensors for SARS-CoV-2 3CLpro Inhibitor DiscoverySemantic proximity Read

van't Hoff enthalpies without baselines.Semantic proximity Read