Article
van't Hoff enthalpies without baselines.
Protein science : a publication of the Protein Society - 1 Jul 2000
John D M, Weeks K M
Abstract excerpt
Analysis of thermal melting curves represents one important approach for evaluating protein stability and the consequences of amino acid substitution on protein structure. By use of the van't Hoff relationship, the differential melting curve can be robustly fit to only three parameters, two of which are the underlying physical constants of melting temperature (Tm) and van't Hoff enthalpy (deltaHvH). Calculated Tm...
Topics
- Cytochrome c Group
- Models, Chemical
- Mutation
- Protein Denaturation
- Proteins
- Temperature
- Thermodynamics