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Orthogonal dual reporter-based gain-of-signal assay for probing SARS-CoV-2 3CL protease activity in living cells: inhibitor identification and mutation investigation.

Emerging microbes & infections - 1 Dec 2023

Bei Zhu-Chun, Yu Huanhuan, Wang Hong, Li Qingyun, Wang Baogang, Zhang Dongna, Xu Likun, Zhao Liangliang, Dong Shuwei, Song Yabin

Abstract excerpt

ABSTRACTThe main protease (3-chymotrypsin-like protease, 3CLpro) of SARS-CoV-2 has become a focus of anti-coronavirus research. Despite efforts, drug development targeting 3CLpro has been hampered by limitations in the currently available activity assays. Additionally, the emergence of 3CLpro mutations in circulating SARS-CoV-2 variants has raised concerns about potential resistance. Both emphasize the need for a...

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Identifiers

PMID: 37144395
DOI: 10.1080/22221751.2023.2211688
PMCID: PMC10187092

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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