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Droplet Digital PCR-Based Detection and Quantification of GyrA Thr-86-Ile Mutation Based Fluoroquinolone-Resistant Campylobacter jejuni.

Microbiology spectrum - 27 Apr 2022

Luo Yi, Zhang Wenting, Cheng Yiluo, Lu Qin, Guo Yunqing, Wen Guoyuan, Shao Huabin, Cheng Zhenyu, Luo Qingping, Zhang Tengfei

Abstract excerpt

Fluoroquinolone (FQ)-resistant Campylobacter jejuni is a serious problem worldwide that limits effective treatment of infections. The traditional detection method depends on bacterial isolation and MIC testing, or traditional PCR, which is time-consuming and hard to identify the FQ-resistant C. jejuni in a high abundance wild-type background. This study aimed to develop a rapid and accurate ddPCR assay to detect...

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Identifiers

PMID: 35412390
DOI: 10.1128/spectrum.02769-21
PMCID: PMC9045142

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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