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Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants.

Scientific reports - 29 Jul 2022

Juul Sebastian, Spiegelhauer Malene Roed, Petersen Mette Neve, Flugt Katharina Kirkegaard, Hansen Nikolaj Vestergaard, Larsen Helene, Jensen Per Bo, Christensen Ulf Bech, Petersen Rasmus Koefoed, Friis-Hansen Lennart

Abstract excerpt

Reverse transcription quantitative PCR (RT-qPCR) assays are gold standard in diagnosing SARS-CoV-2 infection and play a major role in viral subtyping for rapid detection and monitoring of important mutations, containing the spread of new virus variants. We wanted to compare RT-qPCR melting curve analysis assays to Sanger Sequencing for detection of variants within the SARS-CoV-2 spike glycoprotein and examined...

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PMID: 35906388
DOI: 10.1038/s41598-022-17339-0
PMCID: PMC9338320

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