Article
HLA-A, -B, -C genotyping and expression in human nonlymphoid tumor cell lines.
Journal of immunotherapy (Hagerstown, Md. : 1997) - 1 Jan 1999
Delfino L, Ciccarelli G, Bini D, Morabito A, Pozzi S, Martayan A, Giorda E, Setini A, Fraioli R, Giacomini P, Ferrara G B
Abstract excerpt
A combination of molecular genotyping and protein biochemistry methods was used to assess the HLA-A, -B, -C genotyping and expression of six tumor cell lines. Four cell lines had been previously HLA typed by conventional serologic methods. Two could not be typed by serology because deficient in t...
Topics
- Alleles
- Antibodies, Monoclonal
- Cell Membrane
- Flow Cytometry
- Gene Expression
- Genotype
- HLA Antigens
- HLA-A Antigens
- HLA-B Antigens
- HLA-C Antigens
- Histocompatibility Testing
- Humans
- Isoelectric Focusing
- Polymerase Chain Reaction
- Sensitivity and Specificity