Article
EmrE, a small Escherichia coli multidrug transporter, protects Saccharomyces cerevisiae from toxins by sequestration in the vacuole.
Journal of bacteriology - 1 Feb 1999
Yelin R, Rotem D, Schuldiner S
Abstract excerpt
In this report we describe the functional expression of EmrE, a 110-amino-acid multidrug transporter from Escherichia coli, in the yeast Saccharomyces cerevisiae. To allow for phenotypic complementation, a mutant strain sensitive to a series of cationic lipophilic drugs was first identified. A he...
Topics
- 1-Methyl-4-phenylpyridinium
- Acriflavine
- Amino Acid Sequence
- Antiporters
- Bacterial Proteins
- Carrier Proteins
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Ethidium
- Genetic Complementation Test
- Kinetics
- Liposomes
- Membrane Proteins
- Models, Molecular
- Molecular Sequence Data
- Neurotoxins
- Paraquat