Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase.

Abstract excerpt

Subtiligase, a double mutant of subtilisin, has been shown to be capable of joining together two unprotected peptide fragments, namely an activated peptide ester and a second peptide with a free N-terminal amino group. Inside cells, inteins are know to join peptide chains (exteins) by self-extrusion. The SC VMA1 intein was modified to undergo only in vitro N-terminal cleavage in the presence of small nucleophilic...

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