Article
Use of benzyl mercaptan for direct preparation of long polypeptide benzylthio esters as substrates of subtiligase.
Biochemical and biophysical research communications - 8 Jan 1999
Welker E, Scheraga H A
Abstract excerpt
Subtiligase, a double mutant of subtilisin, has been shown to be capable of joining together two unprotected peptide fragments, namely an activated peptide ester and a second peptide with a free N-terminal amino group. Inside cells, inteins are know to join peptide chains (exteins) by self-extrus...
Topics
- Benzyl Compounds
- Escherichia coli
- Mutation
- Peptide Synthases
- Peptides
- Substrate Specificity
- Subtilisins
- Sulfhydryl Compounds