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Article

A redox trap to augment the intein toolbox.

Biotechnology and bioengineering - 1 Jun 2013

Callahan Brian P, Stanger Matthew, Belfort Marlene

Abstract excerpt

The unregulated activity of inteins during expression and consequent side reactions during work-up limits their widespread use in biotechnology and chemical biology. Therefore, we exploited a mechanism-based approach to regulate intein autocatalysis for biotechnological application. The system, inspired by our previous structural studies, is based on reversible trapping of the intein's catalytic cysteine residue...

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Identifiers

PMID: 23280506
DOI: 10.1002/bit.24821
PMCID: PMC3718494

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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