Article
Intermolecular disulfide bonds between unpaired cysteines retard the C-terminal trans-cleavage of Npu DnaE.
Enzyme and microbial technology - 1 Nov 2018
Xu Yanran, Zhang Lei, Ma Buyong, Hu Lifu, Lu Huili, Dou Tonglu, Chen Junsheng, Zhu Jianwei
Abstract excerpt
Npu DnaE is a naturally occurred split intein possessing robust trans-splicing activity and could be engineered to perform rapid C-terminal cleavage module by a single mutation D118G. Unfortunately, however, for this modified selfcleaving module, reducing agents were needed to trigger the rapid cleavage, which prevents the utilization in purification of disulfide bonds containing recombinant proteins. In this...
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