Characterization of tTA and its functional domain in tetracycline repressor-mediated gene repression system.

doi:10.1007/BF02975295

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Article

Characterization of tTA and its functional domain in tetracycline repressor-mediated gene repression system.

Archives of pharmacal research - 1 Jun 1998

Kim H J, Kim K H

Abstract excerpt

To elucidate of role(s) of tTA as a repressor in the tTA-mediated gene repression system, we introduced mutations into the acidic domain of VP16 and examined the effects of such various mutations. In the transient repression experiment, a region containing 34 amino acids of the activation domain...

Topics

Blotting, Western
Fibroblasts
Glucuronidase
Humans
Mutation
Plasmids
Promoter Regions, Genetic
R Factors
Repressor Proteins
Tetracycline

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