Article
[The cloning and expression of the gene for beta-galactosidase from Candida pseudotropicalis yeasts in Saccharomyces cerevisiae cells].
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) - 1 Jan 2000
Tretiak K A, Zakal'skiĭ A E, Gudz' S P
Abstract excerpt
The gene of beta-galactosidase of lactose-assimilating yeast Candida pseudotropicalis was cloned in pG2 and pBG2-3 hybrid shuttle vectors and expressed in Saccharomyces cerevisiae laboratory strains under the control of own promoter. The plasmids were able to replicate autonomously with relative...
Topics
- Candida
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Lactose
- Phenotype
- Plasmids
- Saccharomyces cerevisiae
- Transformation, Genetic
- beta-Galactosidase
