Article
[The cloning and expression of the beta-galactosidase gene of Candida pseudotropicalis yeasts in Escherichia coli cells].
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) - 1 Jan 2000
Tretiak K A, Zakal'skiĭ A E, Gudz' S P
Abstract excerpt
The gene encoding the beta-galactosidase of the yeast Candida pseudotropicalis was cloned on YEp13 shuttle vector as the XhoI-fragment of chromosomal DNA of about 9.5 kb. SalGI-fragment of 7.5 kb with the beta-galactosidase gene was subcloned from the pG2 hybrid plasmid obtained into the pBR322 p...
Topics
- Candida
- Cloning, Molecular
- DNA, Bacterial
- DNA, Fungal
- Escherichia coli
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Vectors
- Mutation
- Plasmids
- Transformation, Bacterial