Article
Limited proteolysis of a trypanosomal hypoxanthine phosphoribosyltransferase yields crystals that diffract X-rays to near atomic resolution.
Biochimica et biophysica acta - 10 Nov 1998
Nieves-Alicea R, Focia P J, Craig S P, Eakin A E
Abstract excerpt
Two crystal forms of the hypoxanthine phosphoribosyltransferase from Trypanosoma cruzi were grown and characterized. Proteolytic modification at the C-terminus of the recombinant enzyme yielded monoclinic crystals that diffract X-rays to higher resolution than the original, trigonal crystal form....
Topics
- Amino Acid Sequence
- Animals
- Crystallization
- Crystallography
- Crystallography, X-Ray
- Endopeptidases
- Escherichia coli
- Hypoxanthine Phosphoribosyltransferase
- Mass Spectrometry
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Recombinant Proteins
- Trypanosoma cruzi