High level multiplex genotyping by MALDI-TOF mass spectrometry.

doi:10.1038/4328

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Article

High level multiplex genotyping by MALDI-TOF mass spectrometry.

Nature biotechnology - 1 Dec 1998

Ross P, Hall L, Smirnov I, Haff L

Abstract excerpt

A primer extension assay is used to perform highly multiplexed genotyping of single nucleotide polymorphisms (SNPs) present in genomic DNA amplified by a multiplex PCR. The assay uses matrix-assisted laser desorption ionization time-of-flight mass spectrometry to accurately measure the masses of...

Topics

Base Sequence
DNA
DNA Primers
Genotype
Humans
Molecular Weight
Polymerase Chain Reaction
Reference Values
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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