Article
New ligands for HLA DRB1*0301 by random selection of favourable amino acids ranked by competition studies with undecapeptide amide sublibraries.
Journal of immunological methods - 1 Oct 1998
Jung C, Kalbus M, Fleckenstein B, Melms A, Jung G, Wiesmüller K H
Abstract excerpt
An efficient screening procedure for the identification of high affinity HLA class II ligands and their binding pattern has been established to characterize peptide specificities for the HLA allele DRB1*0301. The method is based on the screening of 209 synthetic undecapeptide amide sublibraries O...
Topics
- Alleles
- Amides
- Amino Acid Sequence
- Amino Acids
- Apolipoproteins B
- Binding Sites
- Binding, Competitive
- Biotinylation
- Databases, Factual
- Enzyme-Linked Immunosorbent Assay
- HLA-DR Antigens
- HLA-DRB1 Chains
- Humans
- Inhibitory Concentration 50
- Internet
- Ligands
- Models, Biological
- Oligopeptides