Article
Omega-oxidation impairs oxidizability of polyenoic fatty acids by 15-lipoxygenases: consequences for substrate orientation at the active site.
The Biochemical journal - 1 Dec 1998
Ivanov I, Schwarz K, Holzhütter H G, Myagkova G, Kühn H
Abstract excerpt
During oxygenation by 15-lipoxygenases, polyenoic fatty acids are bound at the active site in such a way that the omega-terminus of the fatty acids penetrates into the substrate binding pocket. In contrast, for arachidonic acid 5-lipoxygenation, an inverse head to tail orientation has been sugges...
Topics
- Animals
- Arachidonate 15-Lipoxygenase
- Arachidonic Acids
- Arginine
- Binding Sites
- Fatty Acids, Unsaturated
- Humans
- Mutation
- Polyenes
- Rabbits
- Substrate Specificity