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Article

Evidence for a role of helix IV in connecting cation- and sugar-binding sites of Escherichia coli melibiose permease.

Biochemistry - 18 Apr 2000

Cordat E, Leblanc G, Mus-Veteau I

Abstract excerpt

To improve the structural organization model of melibiose permease, we assessed the individual contributions of the N-terminal tryptophans to the transporter fluorescence variations induced by the binding of cations and beta-configured sugars, by replacement of the six N-terminal tryptophans by phenylalanines and the study of the signal changes. Only two mutations, W116F located in helix IV and W128F located in...

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PMID: 10757998
DOI: 10.1021/bi991852i

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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