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Article

Development of an alpha-complementation system for mycobacterial promoter analysis.

Gene - 14 Sept 1998

Knipfer N, Nooruddin L, Shrader T E

Abstract excerpt

alpha-Complementation is the restoration of beta-galactosidase (beta-Gal) activity to a lacZ mutant via expression of a differentially altered mutant. Here we report the development of an alpha-complementation system for the mycobacteria. Mycobacterium smegmatis alpha-acceptor strains were constructed employing both a novel lacZ alpha-acceptor allele, termed lacZDeltaPvuII, and the widely exploited alpha-acceptor...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 9795143
DOI: 10.1016/s0378-1119(98)00361-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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