Development of an alpha-complementation system for mycobacterial promoter analysis.

doi:10.1016/s0378-1119(98)00361-8

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Article

Development of an alpha-complementation system for mycobacterial promoter analysis.

Gene - 14 Sept 1998

Knipfer N, Nooruddin L, Shrader T E

Abstract excerpt

alpha-Complementation is the restoration of beta-galactosidase (beta-Gal) activity to a lacZ mutant via expression of a differentially altered mutant. Here we report the development of an alpha-complementation system for the mycobacteria. Mycobacterium smegmatis alpha-acceptor strains were constr...

Topics

Alleles
Base Sequence
Chaperonin 60
Escherichia coli
Genetic Complementation Test
Molecular Sequence Data
Mycobacterium
Mycobacterium bovis
Mycobacterium smegmatis
Oligodeoxyribonucleotides
Plasmids
Promoter Regions, Genetic
Restriction Mapping
beta-Galactosidase

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