Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coli.

doi:10.1016/0378-1119(93)90171-x

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Article

Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coli.

Gene - 30 Nov 1993

Smiley B K, Minion F C

Abstract excerpt

Expression of mycoplasma sequences in Escherichia coli is often hindered by an unusual mycoplasmal codon usage pattern: the UGA stop codon is utilized for tryptophan. This may result in the truncation of cloned proteins and may prevent the detection of products of many cloned genes. To circumvent...

Topics

Adhesins, Bacterial
Bacterial Proteins
Base Sequence
Cloning, Molecular
Codon
DNA Primers
Epitopes
Escherichia coli
Molecular Sequence Data
Mutation
Mycoplasma pneumoniae
Plasmids
RNA, Transfer, Trp
Recombinant Proteins
Terminator Regions, Genetic
Tryptophan

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