Article
Deletion of six open reading frames from the left arm of chromosome IV of Saccharomyces cerevisiae.
Yeast (Chichester, England) - 15 Sept 1999
Tuller G, Prein B, Jandrositz A, Daum G, Kohlwein S D
Abstract excerpt
The construction of six deletion mutants of Saccharomyces cerevisiae and their basic phenotypic characterization are described. Open reading frames YDL148c, YDL109c, YDL021w, YDL019c, YDL018c and YDL015c from the left arm of chromosome IV were deleted using a polymerase chain reaction (PCR)-based disruption technique, introducing the kanMX4 resistance marker into the respective genes. Gene replacement cassettes...
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