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Article

A mutation Ser213/Asn in the hexokinase 1 from Schizosaccharomyces pombe increases its affinity for glucose.

Biochemical and biophysical research communications - 29 Oct 1998

Petit T, Herrero P, Gancedo C

Abstract excerpt

Alignment of amino acids of the region implicated in glucose binding from a series of hexokinases showed that Schizosaccharomyces pombe hexokinase 1 had a Ser residue in a place where all other kinases had an Asn. We changed an AGT codon to AAT to place an Asn in the Ser213 position. This mutation decreased Km for glucose from 9.4 mM to 1.6 mM and the ratio Vmax (Fructose)/Vmax (Glucose) from 5 to 2.5. Also the...

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Identifiers

PMID: 9790975
DOI: 10.1006/bbrc.1998.9538

Genes

AGT

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