Article
A mutation Ser213/Asn in the hexokinase 1 from Schizosaccharomyces pombe increases its affinity for glucose.
Biochemical and biophysical research communications - 29 Oct 1998
Petit T, Herrero P, Gancedo C
Abstract excerpt
Alignment of amino acids of the region implicated in glucose binding from a series of hexokinases showed that Schizosaccharomyces pombe hexokinase 1 had a Ser residue in a place where all other kinases had an Asn. We changed an AGT codon to AAT to place an Asn in the Ser213 position. This mutatio...
Topics
- Amino Acid Sequence
- Asparagine
- Binding Sites
- Conserved Sequence
- Fermentation
- Glucose
- Hexokinase
- Isoenzymes
- Kinetics
- Molecular Sequence Data
- Mutation
- Oxygen Consumption
- Recombinant Proteins
- Saccharomyces cerevisiae
- Schizosaccharomyces
- Sequence Homology, Amino Acid
- Serine