Article
Comparison of methods for identifying transcription units and transcription map of the Werner syndrome gene region.
Genomics - 15 Sept 1998
Hisama F M, Oshima J, Yu C E, Fu Y H, Mulligan J, Weissman S M, Schellenberg G D
Abstract excerpt
To isolate a human disease gene by positional cloning, a critical step is the identification of candidate genes from a targeted genomic region. We used cDNA selection, exon trapping, and genomic sequencing to identify 12 transcription units from a 1.4-Mb genomic region containing the Werner syndr...
Topics
- Blotting, Northern
- Chromosome Mapping
- Cloning, Molecular
- Expressed Sequence Tags
- Humans
- Mutation
- Sequence Analysis, DNA
- Transcription, Genetic
- Werner Syndrome