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Article

Time-resolved fluorescence study of azurin variants: conformational heterogeneity and tryptophan mobility.

Biophysical journal - 1 Nov 1998

Kroes S J, Canters G W, Gilardi G, van Hoek A, Visser A J

Abstract excerpt

Time-resolved fluorescence and time resolved fluorescence anisotropy studies have been performed on wild-type azurin from Pseudomonas aeruginosa and two variants to study the mobility of Trp48. The two azurin variants in which the microenvironment of Trp48 was changed comprised the single mutations Ile7Ser and Phe110Ser. The experiments were performed on the holo-Cu(I), holo-Cu(II), and apo- forms at various pH...

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Identifiers

PMID: 9788939
DOI: 10.1016/S0006-3495(98)77688-X
PMCID: PMC1299918

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Source receipt: pubmed, retrieved 7 Sept 2026.

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