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Article

Sensitive detection of the redox state of copper proteins using fluorescence.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry - 1 Oct 2005

Schmauder Ralf, Alagaratnam Sharmini, Chan Chris, Schmidt Thomas, Canters Gerard W, Aartsma Thijs J

Abstract excerpt

The blue copper protein azurin from Pseudomonas aeruginosa has been covalently labelled with the fluorescing dye Cy5. The optical spectrum of the azurin changes markedly with its redox state. These changes are reflected in the fluorescence intensity of the dye through fluorescence resonance energy transfer (FRET). This provides a sensitive way to monitor biological redox events. The method shown to work in the...

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PMID: 16158291
DOI: 10.1007/s00775-005-0020-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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