Article
Effects of cavity-forming mutations on the internal dynamics of azurin.
Biophysical journal - 1 Feb 2004
Cioni Patrizia, de Waal Ellen, Canters Gerard W, Strambini Giovanni B
Abstract excerpt
The effects of two single-point cavity-forming mutations, F110S and I7S, on the internal dynamics of azurin from Pseudomonas aeruginosa were probed by the phosphorescence emission of Trp-48, deeply buried in the compact hydrophobic core of the macromolecule. Changes in flexibility of the protein...
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