Article
Myoglobin mutants giving the largest geminate yield in CO rebinding in the nanosecond time domain.
Biophysical journal - 1 Nov 1998
Sugimoto T, Unno M, Shiro Y, Dou Y, Ikeda-Saito M
Abstract excerpt
We have measured the rebinding of carbon monoxide (CO) to some distal mutants of myoglobin (Mb) in the time range from 10(-8) to 10(-1) s by flash photolysis, in which the photodissociated CO rebinds to the heme iron without escaping to the solvent water from the protein matrix. We have found that the double mutants [His64-->Val/Val68-->Thr (H64V/V68T) and His64-->Val/Val68-->Ser (H64V/V68S)] have an extremely...
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