Article
CO recombination to human myoglobin mutants in glycerol-water solutions.
Biochemistry - 9 Mar 1993
Balasubramanian S, Lambright D G, Marden M C, Boxer S G
Abstract excerpt
The kinetics of CO recombination to site-specific mutants of human myoglobin have been studied by flash photolysis in the temperature range 250-320 K on the nanosecond to second time scale in 75% glycerol at pH 7. The mutants were constructed to examine specific proposals concerning the roles of Lys 45, Asp 60, and Val 68 in the ligand binding process. It is found that ligand recombination is nonexponential for...
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