Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping.

Nucleic acids research - 1 Nov 1998

Hu Y W, Balaskas E, Kessler G, Issid C, Scully L J, Murphy D G, Rinfret A, Giulivi A, Scalia V, Gill P

Abstract excerpt

A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In the presence of an incomplete set of dNTPs, pfu was found to be extremely discriminative in nucleotide incorporation and proofreading at the initiation step of DNA synthesis, completely preventing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to DNA
Share to DNA Primers

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA, Viral

Share to Genetic Techniques

Share to Genotype

Share to Hepacivirus

Share to Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 9776770
DOI: 10.1093/nar/26.21.5013
PMCID: PMC147926

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping.PMID 9776770Read ↗

Variable fragment length allele-specific polymerase chain reaction (VFLASP), a method for simple and reliable genotyping.Semantic proximity Read ↗

Fluorescent allele-specific PCR (FAS-PCR) improves the reliability of single nucleotide polymorphism screening.Semantic proximity Read ↗

An Assay for Variable Fragment Length Allele-Specific Genotyping.Semantic proximity Read ↗

Polymerase chain reaction amplification of specific alleles: a general method of detection of mutations, polymorphisms, and haplotypes.Semantic proximity Read ↗

Primer-extension preamplified DNA is a reliable template for genotyping.Semantic proximity Read ↗

A multiplexing single nucleotide polymorphism typing method based on restriction-enzyme-mediated single-base extension and capillary electrophoresis.Semantic proximity Read ↗

PCR amplification of specific alleles: rapid detection of known mutations and polymorphisms.Semantic proximity Read ↗

Sequence-based detection of single nucleotide polymorphisms.Semantic proximity Read ↗

New universal primers facilitate Pyrosequencing.Semantic proximity Read ↗

Allele-specific PCR in SNP genotyping.Semantic proximity Read ↗

CalcDalton: a tool for multiplex genotyping primer design for single-base extension reactions using cleavable primers.Semantic proximity Read ↗

Efficient mutagenesis method for producing the templates of single nucleotide polymorphisms.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.