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Article

Primer specific and mispair extension analysis (PSMEA) as a simple approach to fast genotyping.

Nucleic acids research - 1 Nov 1998

Hu Y W, Balaskas E, Kessler G, Issid C, Scully L J, Murphy D G, Rinfret A, Giulivi A, Scalia V, Gill P

Abstract excerpt

A simple method, primer specific and mispair extension analysis (PSMEA) with pfu DNA polymerase was developed for genotyping. PSMEA is based on the unique properties of 3'-->5' exonuclease proofreading activity. In the presence of an incomplete set of dNTPs, pfu was found to be extremely discrimi...

Topics

Base Pair Mismatch
Base Sequence
DNA
DNA Primers
DNA, Viral
DNA-Directed DNA Polymerase
Genetic Techniques
Genotype
Hepacivirus
Humans
Sequence Analysis, DNA
Thalassemia

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Identifiers

PMID: 9776770
DOI: 10.1093/nar/26.21.5013
PMCID: PMC147926

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9776770-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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