Article
Microsatellite allele sizing: difference between automated capillary electrophoresis and manual technique.
BioTechniques - 1 Oct 2001
Delmotte F, Leterme N, Simon J C
Abstract excerpt
By comparing data collected with different automated sequencers and a manual technique (fragment separation in a silver-stained polyacrylamide gel), we found strong discrepancies in allele size of microsatellite loci. To quantify the sizing bias generated by automated capillary electrophoresis, we typed 51 alleles at seven loci andfound that differences between actual (manual) and called (automated) sizing were...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.