Article
Application of automated DNA sizing technology for genotyping microsatellite loci.
Genomics - 1 Dec 1992
Ziegle J S, Su Y, Corcoran K P, Nie L, Mayrand P E, Hoff L B, McBride L J, Kronick M N, Diehl S R
Abstract excerpt
Highly polymorphic microsatellite loci offer great promise for gene mapping studies, but fulfillment of this potential will require substantial improvements in methods for accurate and efficient genotyping. Here, we report a genotyping method based on fluorescently labeled PCR primers and size characterization of PCR products using an automated DNA fragment analyzer. We capitalize on the availability of three...
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