Article
Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex.
Protein expression and purification - 1 Oct 1998
Horan T P, Simonet L, Jacobsen R, Mann M, Haniu M, Wen J, Arakawa T, Kuwamoto M, Martin F
Abstract excerpt
Previously, we have shown that the entire extracellular domain of the granulocyte-colony stimulating factor receptor (sG-CSFr) produced in Chinese hamster ovary (CHO) cells forms a stable complex with its ligand G-CSF, at a stoichiometry of 2:2. A truncated receptor molecule consisting of the cyt...
Topics
- Animals
- CHO Cells
- Cricetinae
- Culture Media, Conditioned
- Gene Expression
- Glycosylation
- Granulocyte Colony-Stimulating Factor
- Humans
- Macromolecular Substances
- Mutagenesis, Site-Directed
- Mutation
- Receptors, Granulocyte Colony-Stimulating Factor
- Transfection