Article
Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex.
Protein expression and purification - 1 Oct 1998
Horan T P, Simonet L, Jacobsen R, Mann M, Haniu M, Wen J, Arakawa T, Kuwamoto M, Martin F
Abstract excerpt
Previously, we have shown that the entire extracellular domain of the granulocyte-colony stimulating factor receptor (sG-CSFr) produced in Chinese hamster ovary (CHO) cells forms a stable complex with its ligand G-CSF, at a stoichiometry of 2:2. A truncated receptor molecule consisting of the cytokine receptor homology domain and N-terminus Ig-like domain (Ig CRH) behaves quite similarly. Both of these forms of...
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