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Article

Lowering the pH optimum of D-xylose isomerase: the effect of mutations of the negatively charged residues.

Molecules and cells - 31 Aug 1998

Cha J, Batt C A

Abstract excerpt

Streptomyces rubiginosus D-xylose isomerase catalyzes the reversible isomerization of D-glucose to D-fructose. The isomerization reaction is maximized in the alkaline region of pH 8.5-8.8. The amino acid residues around two active site histidines (His-54 and His-220) and on the surface of the enzyme were mutated to improve the catalytic efficiency at neutral pH. The mutations have been made by removing the...

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PMID: 9749522

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