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Article

Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis.

Biochimie - 1 Sept 2013

Tishkov Vladimir I, Gusakov Alexander V, Cherkashina Anna S, Sinitsyn Arkady P

Abstract excerpt

Endo-1,4-β-glucanase from Penicillium verruculosum (PvEGIII) belongs to family 12 of glycoside hydrolases (GH12). Analysis of the enzyme 3D model structure showed that the amino acid residue Asp98 may directly affect the pH-profile of enzyme activity since it is located at the distance of hydrogen bond formation from Glu203 that plays the role of a general acid in catalysis. The gene encoding the PvEGIII was...

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Identifiers

PMID: 23774299
DOI: 10.1016/j.biochi.2013.05.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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