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Article

Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts.

Scientific reports - 23 Jan 2018

Yang Jun, Cai Haiming, Liu Jie, Zeng Min, Chen Jiawei, Cheng Qingmei, Zhang Linghua

Abstract excerpt

Alcohol oxidase I (AOX1) promoter is the most popular but strictly-regulated methanol inducible promoter for heterologous protein expression in Pichia pastoris. In recent years, AOX1 promoter libraries have been developed with deletion or insertion methods. The present research manipulated poly (dA:dT) tracts in this promoter to control promoter strength, which hadn't been tried before. There were 34 variants...

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Source

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Identifiers

PMID: 29362428
DOI: 10.1038/s41598-018-19831-y
PMCID: PMC5780452

Genes

AOX1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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