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Article

Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus.

International journal of molecular sciences - 2 Feb 2021

Le Thien-Kim, Ju Su-Bin, Lee Hye-Won, Lee Jin-Young, Oh So-Hyung, Kwon Kil-Koang, Sung Bong-Hyun, Lee Seung-Goo, Yeom Soo-Jin

Abstract excerpt

Methanol dehydrogenase (Mdh), is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy. Engineering of Mdh, especially NAD-dependent Mdh, has thus been actively investigated to enhance methanol conversion. However, its poor catalytic activity and low methanol affinity limit its wider application. In this study, we applied a transcriptional...

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Identifiers

PMID: 33540582
DOI: 10.3390/ijms22031471
PMCID: PMC7867188

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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