Subunit exchange of lens alpha-crystallin: a fluorescence energy transfer study with the fluorescent labeled alphaA-crystallin mutant W9F as a probe.

doi:10.1016/s0014-5793(98)00707-8

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Article

Subunit exchange of lens alpha-crystallin: a fluorescence energy transfer study with the fluorescent labeled alphaA-crystallin mutant W9F as a probe.

FEBS letters - 3 Jul 1998

Sun T X, Akhtar N J, Liang J J

Abstract excerpt

A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6...

Topics

Anilino Naphthalenesulfonates
Crystallins
Energy Transfer
Fluorescent Dyes
Humans
Mutation
Spectrometry, Fluorescence
Tryptophan

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