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Article

A whole-genome analysis of allelic changes in renal cell carcinoma by in-gel competitive reassociation.

Molecular carcinogenesis - 1 Jul 1998

Ohki R, Oishi M, Kiyama R

Abstract excerpt

We applied a differential cloning procedure, the in-gel competitive reassociation (IGCR) method, to clone altered genomic sites from the whole genomes of renal cell carcinoma cells. After four rounds of IGCR, we obtained from two patients libraries enriched 1000- and 2500-fold for differential DNA fragments specific to allelic changes in renal cell carcinoma. In these libraries, we found differential fragments of...

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PMID: 9688141
DOI: 10.1002/(sici)1098-2744(199807)22:3<158::aid-mc3>3.0.co;2-h

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