Article
Determination of the conformations of cAMP receptor protein and its T127L,S128A mutant with and without cAMP from small angle neutron scattering measurements.
The Journal of biological chemistry - 7 Aug 1998
Krueger S, Gorshkova I, Brown J, Hoskins J, McKenney K H, Schwarz F P
Abstract excerpt
Small angle neutron scattering (SANS) measurements were performed on solutions of cAMP receptor protein (CRP) and on solutions of the T127L,S128A double mutant of CRP (CRP*) in D2O K3PO4 buffer containing 0.5 M KCl, in the absence and presence of 3',5' cyclic adenosine monophosphate (cAMP). Energ...
Topics
- Crystallography, X-Ray
- Cyclic AMP
- Cyclic AMP Receptor Protein
- Escherichia coli
- Models, Molecular
- Mutation
- Neutrons
- Protein Conformation
- Protein Structure, Secondary
- Scattering, Radiation
- Transcriptional Activation