Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.

Microbial biotechnology - 1 Mar 2013

Hentschel Eva, Will Cornelia, Mustafi Nurije, Burkovski Andreas, Rehm Nadine, Frunzke Julia

Abstract excerpt

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression...

Topics

Amino Acid Sequence
Bacterial Proteins
Biotechnology
Corynebacterium glutamicum
Gene Expression Regulation, Bacterial
Genes, Reporter
Genetic Variation
Genetic Vectors
Luminescent Proteins
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

Protein Stability

RNA-Binding Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 22938655
DOI: 10.1111/j.1751-7915.2012.00360.x
PMCID: PMC3917462

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.PMID 22938655Read ↗

New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.Semantic proximity Read ↗

Comparison of mutant forms of the green fluorescent protein as expression markers in Chinese hamster ovary (CHO) and Saccharomyces cerevisiae cells.Semantic proximity Read ↗

Construction of Corynebacterium glutamicum for efficient secretion and production of recombinant proteins using the technology of ARTP mutagenesis and fluorescen activated droplet microfluidic screening.Semantic proximity Read ↗

Enhancement of heterologous protein production in Corynebacterium glutamicum via atmospheric and room temperature plasma mutagenesis and high-throughput screening.Semantic proximity Read ↗

Green fluorescent protein variants fold differentially in prokaryotic and eukaryotic cells.Semantic proximity Read ↗

Principles and applications of green fluorescent protein-based biosensors: a mini-review.Semantic proximity Read ↗

Comparative analysis of twin-arginine (Tat)-dependent protein secretion of a heterologous model protein (GFP) in three different Gram-positive bacteria.Semantic proximity Read ↗

Construction of an expression vector that uses the aph promoter for protein expression in Corynebacterium glutamicum.Semantic proximity Read ↗

An improved GFP cloning cassette designed for prokaryotic transcriptional fusions.Semantic proximity Read ↗

YGFP: a spectral variant of GFP.Semantic proximity Read ↗

Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.Semantic proximity Read ↗

Expression and detection of green fluorescent protein (GFP).Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.