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Article

Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.

Microbial biotechnology - 1 Mar 2013

Hentschel Eva, Will Cornelia, Mustafi Nurije, Burkovski Andreas, Rehm Nadine, Frunzke Julia

Abstract excerpt

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression...

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Identifiers

PMID: 22938655
DOI: 10.1111/j.1751-7915.2012.00360.x
PMCID: PMC3917462

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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