Article
A convenient method of DNA extraction from blood anticoagulated with EDTA.
Biochemistry and molecular biology international - 1 Jul 1998
Yokota M, Sindo K, Hiyoshi M, Tsuda I, Tatsumi N
Abstract excerpt
Surplus blood often remains after routine clinical tests using EDTA-anticoagulated samples. To use this blood for DNA analysis, we isolated white cells by adherence to polyethylene terephthalate fibers, which could be stored for several weeks transported by mail after methanol fixation. DNA yield...
Topics
- Adult
- Anticoagulants
- Cell Separation
- DNA
- Edetic Acid
- Filtration
- Gene Amplification
- Genotype
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- Humans
- Leukocytes
- Methanol
- Polyethylene Terephthalates
- Polymerase Chain Reaction